Improved luciferase tagging system for Listeria monocytogenes allows real-time monitoring in vivo and in vitro.
نویسندگان
چکیده
An improved system for luciferase tagging Listeria monocytogenes was developed by constructing a highly active, constitutive promoter. This construct gave 100-fold-higher activity in broth than any native promoter tested and allowed for imaging of lux-tagged L. monocytogenes in food products, during murine infections, and in tumor targeting studies.
منابع مشابه
Novel luciferase reporter system for in vitro and organ-specific monitoring of differential gene expression in Listeria monocytogenes.
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ورودعنوان ژورنال:
- Applied and environmental microbiology
دوره 73 9 شماره
صفحات -
تاریخ انتشار 2007